primary antibody against cited2 sc25375  (Santa Cruz Biotechnology)

Journal: Iranian Journal of Basic Medical Sciences

Article Title: Cited2 inhibited hypoxia-induced proliferation and migration of PASMCs via the TGF-β1/Cited2/PPARγ pathway

doi: 10.22038/IJBMS.2023.74455.16178

Figure Lengend Snippet: Cbp/p300-interacting transactivator with Glu/Asp-rich carboxy-terminal domain 2 (Cited2) expression was down-regulated in hypoxia-induced pulmonary hypertension (HPH) rat models

Article Snippet: A protein-free quick blocking solution (Boster, AR0041) was used to block the membrane at room temperature for 1 hr, and then primary antibodies against Cited2 (1:500, Affinity), anti-PPARγ (1:2000, Proteintech), and anti-β-actin (1:3000, Affinity) were used to incubate the membranes overnight at 4 ° C. On the next day, HRP-labeled secondary antibody (1:5000, Affinity) was used to incubate the membranes at room temperature for 1 hr, and the chemiluminescence method was used for color development.

Techniques: Expressing

Journal: Iranian Journal of Basic Medical Sciences

Article Title: Cited2 inhibited hypoxia-induced proliferation and migration of PASMCs via the TGF-β1/Cited2/PPARγ pathway

doi: 10.22038/IJBMS.2023.74455.16178

Figure Lengend Snippet: Cbp/p300-interacting transactivator with Glu/Asp-rich carboxy-terminal domain 2 (Cited2) expression was down-regulated in pulmonary artery smooth muscle cells (PASMCs) in hypoxia

Article Snippet: A protein-free quick blocking solution (Boster, AR0041) was used to block the membrane at room temperature for 1 hr, and then primary antibodies against Cited2 (1:500, Affinity), anti-PPARγ (1:2000, Proteintech), and anti-β-actin (1:3000, Affinity) were used to incubate the membranes overnight at 4 ° C. On the next day, HRP-labeled secondary antibody (1:5000, Affinity) was used to incubate the membranes at room temperature for 1 hr, and the chemiluminescence method was used for color development.

Techniques: Expressing

Journal: Iranian Journal of Basic Medical Sciences

Article Title: Cited2 inhibited hypoxia-induced proliferation and migration of PASMCs via the TGF-β1/Cited2/PPARγ pathway

doi: 10.22038/IJBMS.2023.74455.16178

Figure Lengend Snippet: Effects of Cbp/p300-interacting transactivator with Glu/Asp-rich carboxy-terminal domain 2 (Cited2) overexpression on pulmonary artery smooth muscle cells (PASMCs)

Article Snippet: A protein-free quick blocking solution (Boster, AR0041) was used to block the membrane at room temperature for 1 hr, and then primary antibodies against Cited2 (1:500, Affinity), anti-PPARγ (1:2000, Proteintech), and anti-β-actin (1:3000, Affinity) were used to incubate the membranes overnight at 4 ° C. On the next day, HRP-labeled secondary antibody (1:5000, Affinity) was used to incubate the membranes at room temperature for 1 hr, and the chemiluminescence method was used for color development.

Techniques: Over Expression

Journal: Iranian Journal of Basic Medical Sciences

Article Title: Cited2 inhibited hypoxia-induced proliferation and migration of PASMCs via the TGF-β1/Cited2/PPARγ pathway

doi: 10.22038/IJBMS.2023.74455.16178

Figure Lengend Snippet: Effects of Cbp/p300-interacting transactivator with Glu/Asp-rich carboxy-terminal domain 2 (Cited2) knockdown on pulmonary artery smooth muscle cells (PASMCs)

Article Snippet: A protein-free quick blocking solution (Boster, AR0041) was used to block the membrane at room temperature for 1 hr, and then primary antibodies against Cited2 (1:500, Affinity), anti-PPARγ (1:2000, Proteintech), and anti-β-actin (1:3000, Affinity) were used to incubate the membranes overnight at 4 ° C. On the next day, HRP-labeled secondary antibody (1:5000, Affinity) was used to incubate the membranes at room temperature for 1 hr, and the chemiluminescence method was used for color development.

Techniques: Knockdown

Journal: Iranian Journal of Basic Medical Sciences

Article Title: Cited2 inhibited hypoxia-induced proliferation and migration of PASMCs via the TGF-β1/Cited2/PPARγ pathway

doi: 10.22038/IJBMS.2023.74455.16178

Figure Lengend Snippet: Cbp/p300-interacting transactivator with Glu/Asp-rich carboxy-terminal domain 2 (Cited2) reversed Transforming growth factor-beta1 (TGF-β1)-induced proliferation and migration of pulmonary artery smooth muscle cells (PASMCs)

Article Snippet: A protein-free quick blocking solution (Boster, AR0041) was used to block the membrane at room temperature for 1 hr, and then primary antibodies against Cited2 (1:500, Affinity), anti-PPARγ (1:2000, Proteintech), and anti-β-actin (1:3000, Affinity) were used to incubate the membranes overnight at 4 ° C. On the next day, HRP-labeled secondary antibody (1:5000, Affinity) was used to incubate the membranes at room temperature for 1 hr, and the chemiluminescence method was used for color development.

Techniques: Migration

Journal: Iranian Journal of Basic Medical Sciences

Article Title: Cited2 inhibited hypoxia-induced proliferation and migration of PASMCs via the TGF-β1/Cited2/PPARγ pathway

doi: 10.22038/IJBMS.2023.74455.16178

Figure Lengend Snippet: Peroxisome proliferatoractivated receptor gamma (PPARγ) cooperates with Cbp/p300-interacting transactivator with Glu/Asp-rich carboxy-terminal domain 2 (Cited2) to inhibit the proliferation and migration of pulmonary artery smooth muscle cells (PASMCs)

Article Snippet: A protein-free quick blocking solution (Boster, AR0041) was used to block the membrane at room temperature for 1 hr, and then primary antibodies against Cited2 (1:500, Affinity), anti-PPARγ (1:2000, Proteintech), and anti-β-actin (1:3000, Affinity) were used to incubate the membranes overnight at 4 ° C. On the next day, HRP-labeled secondary antibody (1:5000, Affinity) was used to incubate the membranes at room temperature for 1 hr, and the chemiluminescence method was used for color development.

Techniques: Migration